cardiac fibroblast growth medium (Cell Applications Inc)
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Cell Applications Inc
cardiac fibroblast growth medium
Cardiac Fibroblast Growth Medium, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 94/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cardiac+fibroblast+growth+medium/Human+Cardiac+Fibroblast+Media/pmc12650109-40-14-18
Average 94 stars, based on 38 article reviews
Cardiac Fibroblast Growth Medium, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 94/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cardiac+fibroblast+growth+medium/Human+Cardiac+Fibroblast+Media/pmc12650109-40-14-18
Average 94 stars, based on 38 article reviews
cardiac fibroblast growth medium - by Bioz Stars,
2026-09
94/100 stars
Images
1) Product Images from "Factors Released by Polarized Neutrophil-like Cells Modulate Cardiac Fibroblast Phenotype and Limit the Inflammatory Response After Myocardial Infarction"
Article Title: Factors Released by Polarized Neutrophil-like Cells Modulate Cardiac Fibroblast Phenotype and Limit the Inflammatory Response After Myocardial Infarction
Journal: Biomedicines
doi: 10.3390/biomedicines13112829
Figure Legend Snippet: Impact of cell culture conditions on human cardiac fibroblasts (CFs) morphology and phenotype. ( A ) Morphology of CFs after 48h in conventional 2D culture ( a , c ) and 3D culture on ARdH hydrogel ( b , d ), as determined by F-actin labeling with phalloidin (red) and DAPI nuclear staining (cyan). The scale bar indicates 200 µm for ( a , b ), and 50 µm for ( c , d ). ( B ) Cell viability of CFs after 48 h in 2D and 3D culture, as determined by MTT assay; ( C ) MCP-1 released in conditioned media by CFs cultured in 2D and 3D conditions determined by ELISA assay; ( D ) mRNA expression of α-SMA, MCP-1, and IL-1β in CFs isolated from 3D-hydrogel or 2D culture after 48 h. ( E ) α-SMA protein expression in CFs from aortic root-derived hydrogel (ARdH) or 2D culture, determined by Western Blot; n = 3, * p < 0.05. Data is represented as mean ± SEM.
Techniques Used: Cell Culture, Labeling, Staining, MTT Assay, Enzyme-linked Immunosorbent Assay, Expressing, Isolation, Derivative Assay, Western Blot
Figure Legend Snippet: Profile of inflammatory mediators and of matrix metalloproteinases (MMP) following a 24 h indirect co-culture of human CFs grown on ARdH hydrogel with control granulocytes (CfN), or their pro-inflammatory (N1) or anti-inflammatory (CfN2) subtypes. ( A ) mRNA level of inflammatory cytokines and chemokines in CFs grown on ARdH hydrogel and indirectly interacted with granulocytes subsets, determined by qPCR—normalized to B2M and represented as fold change compared with C (Cf only); ( B ) Levels of secreted inflammatory cytokines and chemokines in conditioned media from indirect co-cultures as evaluated by ELISA assay; ( C ) IL-1β protein level in CFs lysates following indirect interaction with neutrophil-like cell subtypes, as determined by Western Blot assay and normalized to β-actin; ( D ) Metalloproteases MMP-1, -2, -3, -9 and -13 mRNA expression in CFs indirectly co-cultured with neutrophil-like subtypes; ( E ) Concentration of soluble MMP-1 and MMP-9 quantified in conditioned media as evaluated by ELISA assay; ( F , G ) Protein expression of MMP-9/-1 in CFs following indirect interaction, as evaluated by Western Blot and normalized to β-actin; ( H ) The enzymatic activity of gelatinase MMP-9 in the conditioned media assessed by SDS-PAGE gelatine zymography; ( I ) Intracellular signaling: MAPKs (p38 and ERK1/2) and NF-κB phosphorylation in human CFs following indirect interaction with neutrophil-like cell subtypes; ( J ) Proliferative capacity of primary mouse CFs assessed using the xCelligence system: C+—fibroblasts exposed to DMEM/F12 with 10%FBS and C-—fibroblasts exposed to DMEM/F12 without FBS; N1/N2—fibroblasts exposed to mouse N1/N2 conditioned media; ( K ) Cell index after ~3 days of exposure to N1/N2 conditioned media. n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001 Data is represented as mean ± SEM.
Techniques Used: Co-Culture Assay, Control, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Cell Culture, Concentration Assay, Activity Assay, SDS Page, Zymography, Phospho-proteomics
Related Articles
Cell Culture:Article Title: Temporal expression and spatial distribution of the proteoglycan versican during cardiac fibrosis development Article Snippet: Fetal human cardiac fibroblasts (CFs) (Cell Applications, CA, #306-05F) and primary human cardiomyocytes (CMs) (PromoCell, Germany, #C-12810) were used for in vitro experiments. .. CFs were cultured in Article Title: Temporal expression and spatial distribution of the proteoglycan versican during cardiac fibrosis development Article Snippet: The cytokines were purchased from R&D systems (MN). .. For mechanical stretching of CFs, we used 6-well Bioflex® culture plates (Dunn Labortechnik, Asbach, Germany, #BF-3001U) coated with fibronectin bovine plasma (Merck Sigma, Darmstadt, Germany, #F1141) at a concentration of 2 μg/ml for 24 h. The cells were cultured at a concentration of 3.5 × 10 5 cells/well for 24 h at 37°C in Article Title: Acetylcholine-loaded nanoparticles protect against doxorubicin-induced toxicity in in vitro cardiac spheroids. Article Snippet: Doxorubicin (DOX) is widely used in chemotherapy, yet it significantly contributes to heart failureassociated death.. Acetylcholine (ACh) is cardioprotective by enhancing heart rate variability and reducing mitochondrial dysfunction and inflammation.. Nonetheless, the protective role of ACh in countering DOX-induced cardiotoxicity (DIC) remains underexplored as current approaches to increasing ACh levels are invasive and unsafe for patients. Article Title: Cardiomyocyte intercellular signalling increases oxidative stress and reprograms the global‐ and phospho‐proteome of cardiac fibroblasts Article Snippet: .. Human primary cardiac fibroblasts were obtained from Sigma‐Aldrich (306‐5a, lot: 3357; atrial, single donor: male, 26 years old) and cultured on flasks (coated with 1% gelatin (bovine skin Type B, Sigma‐Aldrich, 9000‐70‐8) overnight) in media consisting of 50% Article Title: Temporal expression and spatial distribution of the proteoglycan versican during cardiac fibrosis development. Article Snippet: Human cardiac cells and cytokine treatment Fetal human cardiac fibroblasts (CFs) (Cell Applications, CA, #306- 05F) and primary human cardiomyocytes (CMs) (PromoCell, Germany, #C-12810) were used for in vitro experiments. .. CFs were cultured in Article Title: Temporal expression and spatial distribution of the proteoglycan versican during cardiac fibrosis development. Article Snippet: .. For mechanical stretching of CFs, we used 6-well Bioflex® culture plates (Dunn Labortechnik, Asbach, Germany, #BF-3001U) coated with fibronectin bovine plasma (Merck Sigma, Darmstadt, Germany, #F1141) at a concentration of 2 μg/ml for 24 h. The cells were cultured at a concentration of 3.5 × 105 cells/well for 24 h at 37◦C in Clinical Proteomics:Article Title: Temporal expression and spatial distribution of the proteoglycan versican during cardiac fibrosis development Article Snippet: The cytokines were purchased from R&D systems (MN). .. For mechanical stretching of CFs, we used 6-well Bioflex® culture plates (Dunn Labortechnik, Asbach, Germany, #BF-3001U) coated with fibronectin bovine plasma (Merck Sigma, Darmstadt, Germany, #F1141) at a concentration of 2 μg/ml for 24 h. The cells were cultured at a concentration of 3.5 × 10 5 cells/well for 24 h at 37°C in Article Title: Temporal expression and spatial distribution of the proteoglycan versican during cardiac fibrosis development. Article Snippet: .. For mechanical stretching of CFs, we used 6-well Bioflex® culture plates (Dunn Labortechnik, Asbach, Germany, #BF-3001U) coated with fibronectin bovine plasma (Merck Sigma, Darmstadt, Germany, #F1141) at a concentration of 2 μg/ml for 24 h. The cells were cultured at a concentration of 3.5 × 105 cells/well for 24 h at 37◦C in Concentration Assay:Article Title: Temporal expression and spatial distribution of the proteoglycan versican during cardiac fibrosis development Article Snippet: The cytokines were purchased from R&D systems (MN). .. For mechanical stretching of CFs, we used 6-well Bioflex® culture plates (Dunn Labortechnik, Asbach, Germany, #BF-3001U) coated with fibronectin bovine plasma (Merck Sigma, Darmstadt, Germany, #F1141) at a concentration of 2 μg/ml for 24 h. The cells were cultured at a concentration of 3.5 × 10 5 cells/well for 24 h at 37°C in Article Title: Temporal expression and spatial distribution of the proteoglycan versican during cardiac fibrosis development. Article Snippet: .. For mechanical stretching of CFs, we used 6-well Bioflex® culture plates (Dunn Labortechnik, Asbach, Germany, #BF-3001U) coated with fibronectin bovine plasma (Merck Sigma, Darmstadt, Germany, #F1141) at a concentration of 2 μg/ml for 24 h. The cells were cultured at a concentration of 3.5 × 105 cells/well for 24 h at 37◦C in |